Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.

Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

414.32

C₁₈H₁₆BrN₅S

298-93-1

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.