生物活性 | |||||||||||||||||
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体外研究 (In Vitro) |
CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
557.46 |
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Formula |
C58H38N2O22 |
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CAS 号 | |||||||||||||||||
中文名称 |
5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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溶解性数据 |
In Vitro:
DMSO : 50 mg/mL (89.69 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百 *以上所有助溶剂都可在 MCE 网站选购。
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参考文献 |
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