小分子抑制剂

Small molecule inhibitor

CFSE

货号:HY-D0938
规格:
名称:Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester

产品详情

生物活性

CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division[1][2].

体外研究
(In Vitro)

CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

557.46

Formula

C58H38N2O22

CAS 号
中文名称

5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro: 

DMSO : 50 mg/mL (89.69 mM; Need ultrasonic)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 1.7939 mL 8.9693 mL 17.9385 mL
5 mM 0.3588 mL 1.7939 mL 3.5877 mL
10 mM 0.1794 mL 0.8969 mL 1.7939 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

     

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

     

     
  • 2.

     

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

     

     
*以上所有助溶剂都可在 MCE 网站选购。
参考文献